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Offres d'emplois et de Post-Doc

STAGE de MASTER 2 (6 mois, second semestre)
Un stage de 6 mois est proposé au laboratoire GEC (Génie Enzymatique et Cellulaire, Université de Picardie Jules Verne, Amiens, France), pour étudier l'interaction des peptides anti-amyloïdogéniques avec des membranes biomimétiques
Unité de Génie Enzymatique et Cellulaire UMR 7025 CNRS, Université de Picardie Jules Verne ; Amiens, France

Titre du projet : fRanchissement de la bArrière hémaTo-encéphalIque pOur la délivraNce de peptides Anti-amyLoïdogènes à activité antibactériEnne (RATIONALE)
Projet : Les maladies neurodégénératives sont majoritairement associées à la présence de peptides et protéines, capables de former des fibres amyloïdes. La plupart des stratégies thérapeutiques consistent à inhiber leur agrégation. Les protéines impliquées dans l’amyloïdogenèse partagent d’importantes similitudes avec les peptides antimicrobiens. Le peptide synthétique QBP1 est un agent prometteur contre l’amyloïdogenèse car il est capable de bloquer l'agrégation de ces protéines sans montrer de toxicité in-vivo. Il montre une forte similarité de séquence avec plusieurs peptides antimicrobiens suggérant une activité antibactérienne jamais étudiée.
Ce projet collaboratif devrait permettre de proposer de nouvelles thérapies efficaces pour lutter contre les maladies neurodégénératives. Il vise à sélectionner des analogues de QBP1 possédant une activité antimicrobienne, capables de franchir la barrière hématoencéphalique et d’inhiber la formation des agrégats amyloïdes. D’un point de vue plus fondamental, il peut permettre de mieux comprendre l’importance des infections microbiennes dans l’initiation et la progression des maladies neurodégénératives.
Description du travail de stage
Dans ce projet, des dérivés peptidiques de QBP1 seront étudiés dans leur interaction avec des modèles de membranes biologiques, mimant la composition lipidique bactérienne. De multiples techniques seront utilisées (dichroïsme circulaire, fluorescence) avec un accent particulier sur la RMN liquide et solide et les simulations de dynamique moléculaire (MD). Des sondes paramagnétiques seront utilisées pour localiser le peptide dans la membrane et déterminer sa structure.
Admissibilité Le candidat retenu devra avoir une formation solide en biochimie ou en biologie structurale dans le domaine de la chimie, de la biologie et/ou de la physique, être désireux d'apprendre et capable de travailler de manière indépendante et en équipe. Des connaissances théoriques en biochimie, une expérience pratique de base en laboratoire et un niveau intermédiaire en anglais sont obligatoires. Pour ce stage, l'expérience de la bioinformatique ou de la programmation informatique n'est pas obligatoire, mais sera considérée comme un atout. Les autres compétences d’intérêts portent sur l’expérience ou la connaissance de la RMN à l'état solide ou en solution.
Comment postuler Les candidats intéressés pour le poste à Amiens pourront envoyer leurs candidatures à nicola.damelio@u-picardie.fr, catherine.sarazin@u-picardie.fr et francisco.ramos@u-picardie.fr.
- Un curriculum vitae complet détaillant les modules universitaires (relevé de notes des cours des trois dernières années d'études universitaires et leurs notes), le classement obtenu chaque année et le nombre de crédits.
- une lettre de motivation du candidat indiquant clairement les compétences qu'il pense avoir acquises pour mener à bien le projet.
- une lettre de recommandation du responsable de l'équipe d'accueil et / ou dde l’encadrant du stage de master 1.
Les candidats sélectionnés seront convoqués pour un entretien (en anglais ou en français, en fonction de leurs préférences).
Informations utiles / dates Date limite: 15/01/2023 Date de début: mi-janvier/février 2023
 
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Lire les informationsAnnonce déposée le 25/11/2022

Postdoctoral Research Associate in Physical Chemistry (Fixed Term)
Reisner Lab
Department of Chemistry
University of Cambridge
Lensfield Road, Cambridge
CB2 1EW, UK

Applications are invited for a 2-year Postdoctoral Research Associate (PDRA) under the supervision of Professor Erwin Reisner in the Yusuf Hamied Department of Chemistry at the University of Cambridge. The project will support the development of biohybrid systems for the controlled synthesis of organic chemicals at high efficiency using sunlight by combining the strength of synthetic and biological technologies. An integrated platform will be developed that incorporates efficient light harvesting by semiconductors to drive a network of synthetic and biocatalysts to convert carbon dioxide to bespoke complex chemical products. The PDRA will oversee the physical chemistry activities, which involve the in situ characterisation and mechanistic studies of the catalysis networks. Specifically, the PDRA will investigate the material-catalyst interface using a range of techniques, in particular spectroscopy (e.g., infrared and Raman), within a cross-disciplinary team with the ambition to develop direct photon-to-chemical conversion technologies.
The PDRA should have (or be about to obtain) a PhD in Physical Chemistry or a closely related discipline. A strong background in spectroscopy, photochemistry, electrochemistry or catalysis would be required for the position.
Start date: January to October 2023.
 
Site WebAnnonce déposée le 10/11/2022

Postdoctoral Research Associate in Biochemistry (Fixed Term)
Reisner Lab
Department of Chemistry
University of Cambridge
Lensfield Road, Cambridge
CB2 1EW, UK

Applications are invited for a 2-year Postdoctoral Research Associate (PDRA) under the supervision of Professor Erwin Reisner in the Yusuf Hamied Department of Chemistry at the University of Cambridge. The project will support the development of biohybrid systems for the controlled synthesis of organic chemicals at high efficiency using sunlight by combining the strength of synthetic and biological technologies. An integrated platform will be developed that incorporates efficient light harvesting by semiconductors to drive a network of enzymes and engineered live cells to convert carbon dioxide to bespoke complex chemical products. The PDRA will support the biological work of the project within a cross-disciplinary team with the ambition to develop bioelectrochemical and biophotocatalytic technologies to synthesise high value chemicals sustainably.
The PDRA should have (or be about to obtain) a PhD in Biochemistry, Chemical Biology, Microbiology or a closely related discipline. A background in synthetic biology, protein purification or bioelectrochemistry would be desirable for the position.
Start date: January to October 2023.
 
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Postdoctoral Research Associate in Organic Chemistry (Fixed Term)
Reisner Lab
Department of Chemistry
University of Cambridge
Lensfield Road, Cambridge
CB2 1EW, UK

Applications are invited for a 2-year Postdoctoral Research Associate (PDRA) under the supervision of Professor Erwin Reisner in the Yusuf Hamied Department of Chemistry at the University of Cambridge. The project will support the development of the controlled synthesis of organic chemicals at high efficiency using sunlight by combining the strength of synthetic and biological technologies. An integrated platform will be developed that incorporates efficient light harvesting by semiconductors to drive a network of synthetic and biological catalysts to convert CO2 to bespoke complex chemical products. The PDRA will work within a cross-disciplinary team with the ambition to develop direct photon-to-chemical conversion technologies based on integrated catalysis networks to synthesise high value chemicals sustainably from sunlight.
The PDRA should have (or be about to obtain) a PhD in Organic Chemistry or a closely related discipline. A background in the development of synthetic organic methodologies, photochemistry or electrocatalysis would be desirable for the position.
Start date: January to October 2023.
 
Site WebAnnonce déposée le 10/11/2022

PhD Studentship: Organic Chemistry
Reisner Lab
Department of Chemistry
University of Cambridge
Lensfield Road, Cambridge
CB2 1EW, UK

Applications are invited for a 4-year PhD studentship in Organic Chemistry under the supervision of Professor Erwin Reisner in the Yusuf Hamied Department of Chemistry. The project will focus on the development and implementation of photo-flow techniques to enable green and cost-effective methods to perform challenging organic transformations based on inexpensive, robust and easily scalable semiconductor photocatalysts. The goal is to achieve a step-change in automated chemical synthesis, which can be easily adopted by research laboratories and industries. As such, one of the main objectives of the project, alongside organic synthesis, will involve the design and advancement of the photo-flow reactor. The project will also establish various detection and analytical methods to allow for the integration of continuous in-line monitoring and high-throughput techniques. Finally, the use of machine learning for the identification of optimum reaction conditions and the design of automated protocols will be supported by the EPSRC SynTech Centre for Doctoral Training. The project is interdisciplinary and will encompass the expertise of organic chemistry (primary), materials science and chemical engineering.
Applicants must have (or expect to obtain) at least the equivalent of a UK first class Master's degree in chemistry. The studentship will provide a maintenance grant and tuition fees at the UK rate. Non-UK applicants will be considered only if they qualify for the UK fees rate or are able to fund the overseas fees differential.
 
Site WebAnnonce déposée le 10/11/2022

PhD Studentship: Chemistry
Reisner Lab
Department of Chemistry
University of Cambridge
Lensfield Road, Cambridge
CB2 1EW, UK

Applications are invited for a 4-year PhD studentship, starting in April or October 2023, under the supervision of Professor Erwin Reisner in the Yusuf Hamied Department of Chemistry. The project will support the development of the controlled synthesis of organic chemicals at high efficiency using sunlight by combining the strength of synthetic and biological technologies. An integrated platform will be developed that incorporates efficient light harvesting by semiconductors to drive a network of synthetic and biological catalysts to convert carbon dioxide to bespoke complex chemical products. The PhD student will work within a cross-disciplinary team with the ambition to develop direct photon-to-chemical conversion technologies based on a general biohybrid platform to synthesise high value chemicals sustainably from sunlight.
Applicants must have (or expect to obtain) at least the equivalent of a UK first class Master's degree in Chemistry, or related discipline such as Materials Science or Chemical Engineering. The studentship will provide a maintenance grant and full tuition fees.
 
Site WebAnnonce déposée le 10/11/2022

POSTDOCTORAL POSITION at IBMC and IGBMC
Université de Strasbourg


 
Our groups “Viral ribonucleoproteins, incorporation of the genome and assembly” at the Institut de Biologie Moléculaire et Cellulaire (IBMC, Strasbourg) and “Chromatin stability and DNA mobility” at the Institut de Génétique, Biologie Moléculaire et Cellulaire (IGBMC, Illkirch) are searching for a postdoc with experience in STRUCTURAL BIOLOGY to join us on a research project on the role of HIV-1 Pr55Gag precursor in the encapsidation of the viral genomic RNA (gRNA). This project is funded by ANRS (Agence Nationale de Recherches sur le Sida et les hépatites virales).

 
RESEARCH and AIM of the PROJECT
HIV-1 must specifically select and package its gRNA from a large excess of cellular and viral spliced RNAs. Packaging of HIV-1 gRNA is a key step for the production of infectious virus, yet, surprisingly, how HIV-1 preferentially recognizes its gRNA is poorly understood. Deciphering the molecular mechanisms governing HIV-1 gRNA packaging would further the development of innovative antiretroviral strategies and enable the design of improved retroviral vectors that could be potentially useful in the treatment of HIV-1 and other human diseases.
HIV-1 gRNA packaging is thought to be mainly mediated by specific interactions occurring between the viral Pr55Gag precursor and a region in the gRNA, termed Psi. However, to date, high resolution structural information on the Pr55Gag/Psi RNA complex is lacking.
Our groups are interested in the structure of the Pr55Gag precursor of HIV-1 in association with a viral RNA fragment containing the Psi by cryo-EM and X-ray crystallography. We also plan to obtain the structure of the Pr55Gag-Psi complex in the presence of the cellular and viral ligands of the p6 domain of Pr55Gag by the same methods.
This work will contribute to define the structural and biophysical properties governing the encapsidation of HIV-1 gRNA by Pr55Gag precursor.
 
REQUIREMENTS / QUALIFICATIONS
We are looking for a Postdoc with demonstrated expertise in biochemistry, molecular and structural biology. Experience in protein expression and purification, in vitro protein-RNA interaction analyses (e.g. by gel filtration, ITC etc.), preparation of the grids for cryo-EM, and resolution of protein structure (e.g. by X-ray crystallography and/or Cryo-EM) is required. Experience in RNA production is advantageous.
Candidates should have obtained their PhD within the last 5 years, and must be less than 40. They should have published at least one first-author peer-reviewed research paper. Good English communication skills are expected. Great emphasis will be placed on personal qualities such as organization, creativity, motivation, flexibility and ability to work in a team. Candidates should also have a good ability to conduct independent research, and to supervise technicians and students.

 
DURATION & STARTING DATE
The grant is for 3 years. The starting date is 3rd of January 2023. Reviewing of applications will begin immediately and will continue until a suitable candidate is found. Please send your application by e- mail to Serena Bernacchi (s.bernacchi@ibmc-cnrs.unistra.fr).
Please provide a CV and a brief statement of research interests, qualifications and if possible, include at least one letter of recommendation.
 
RESEARCH ENVIRONMENT
Strasbourg is a center for research in Life Sciences and a beautiful and lively city in the east of France, close to Germany. IBMC and IGBMC offer an innovative and scientifically stimulating environment with access to state-of-the-art infrastructure, including equipment for protein biochemistry, and molecular biology and structure biology laboratories, which are part of the French Infrastructure for Integrated Structural Biology (FRISBI).
 
Lire les informationsAnnonce déposée le 10/11/2022

PhD Studentship: Biochemistry
Reisner Lab
Department of Chemistry
University of Cambridge
Lensfield Road, Cambridge
CB2 1EW, UK

Applications are invited for a 4-year PhD studentship, starting in April or October 2023, under the supervision of Professor Erwin Reisner in the Yusuf Hamied Department of Chemistry. The project will support the development of biohybrid systems for the controlled synthesis of organic chemicals at high efficiency using sunlight by combining the strength of synthetic and biological technologies. An integrated platform will be developed that incorporates efficient light harvesting by semiconductors to drive a network of enzymes and engineered live cells to convert carbon dioxide to bespoke complex chemical products. The PhD student will support the biological work within a cross-disciplinary team with the ambition to develop direct photon-to-chemical conversion technologies based on a general biohybrid platform to synthesise high value chemicals sustainably from sunlight.
Applicants must have (or expect to obtain) at least the equivalent of a UK first class Master's degree in Biochemistry, Chemical Biology, Microbiology or related discipline. The studentship will provide a maintenance grant and full tuition fees.
 
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Open postdoc position on structural biology and biophysics of cardiac amyloids
Dept. of Biosciences of University of Milan — Italy

Position for a postdoctoral fellow is open in the two institutions at the Dept. of Biosciences of University of Milan (Italy) and at the Institute for Molecular and Translational Cardiology, at the Research Hospital Policlinico San Donato (Milan, Italy).
 
Candidates may have a solid background in protein biochemistry and biophysics, structural biology (X-ray crystallography and Cryo-EM) of folded/misfolded proteins. Experience in mammalian cell expression and in techniques for ligand binding would be considered as positive assets.

 
The project will deal with structure determination of ex-vivo amyloids by Cryo-EM and biophysics of amyloidogenic proteins under native conditions.
The contract will be at European standards and will last 3-4 years.
Interested candidates should send a CV and a motivation letter to Stefano.ricagno@unimi.it
 
Lire les informationsAnnonce déposée le 17/10/2022

1 PhD position, starting January 2023
65% TVöD E13 (3 years)
on Gauging membrane tension and channel activity with light
within the Cluster of Excellence UniSysCat in the Berlin/Potsdam area

Project description – the job
In this project, we will establish an artificial cell-mimetic system, in which membrane tension is modulated by light using photo- responsive lipids and vectorial transport across the membrane is triggered by photo-induced tension ramp. As a workbench, we will employ micron-sized membrane compartments, namely giant unilamellar vesicles (see figure), in which the mechano-sensitive channel of large conductance (MscL) will be reconstituted. The vesicle membrane will contain photoswitchable lipid the isomerization of which will modulate the membrane tension. Using biophysical approaches based on micropipette aspiration, vesicle electrodeformation, optical microscopy, as well as microfluidics, we will precisely measure the imposed tension needed to gauge the opening of the channel. The established methodology will be applied using both bottom-up and top-down pathways for the preparations of the membrane compartments, namely using giant vesicle swelling from a few lipids (bottom-up) and deriving vesicles from the plasma membrane of cells whereby preserving the molecular protein-lipid variety (top-down). The established methodologies will be introduced to explore the performance of the mechanosensitive channel TRAAK as well.
 
Research environment – the group
The PhD student will join the team of Dr. Rumiana Dimova. Dr. Dimova is a leading expert in the field of membrane biophysics and her international group offers a highly cooperative working environment and cutting-edge experimental facilities. Collaborations with the research group of Prof. Joachim Heberle at the Free University of Berlin will be involved in the project development. The Max Planck Institute of Colloids and Interfaces in Potsdam is a world-renowned research institution that offers interdisciplinary and collaborative research environment at national and international level.
 
Requirements – the candidates
  • MSc or equivalent degree in: (bio)physics / (bio)chemistry / (bio)engineering
  • interest in: physics of biological systems
  • experience with membranes & membrane proteins / microscopy / microfluidics will be an advantage
  • excellent command of English & ability for independent research
Submit your application via email to rumiana.dimova[at]mpikg.mpg.de including:
  • motivation letter: why do you apply for this position, what are your research interests, how do you fit
  • detailed CV
  • university grades transcripts (BSc, MSc)
  • names and email addresses of 2 reference persons ready to give feedback
Your application will be reviewed as soon as it is received. The position will be filled ASAP. Starting time: 1.1.2023
 
The Max Planck Society strives to ensure a workplace that embraces diversity and provides equal opportunities irrespective of the applicants’ gender, nationality or disabilities. The Max Planck Society is committed to increasing the number of individuals with disabilities in its workforce and therefore encourages applications from such qualified individual
 
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Lire les informationsAnnonce déposée le 10/10/2022

POSTDOCTORAL POSITION IN ATOMIC FORCE MICROSCOPY
Complutense University of Madrid, Spain
POSTDOCTORAL POSITION IN ATOMIC FORCE MICROSCOPY (Starting date: Jan 2023)

Strongly motivated candidates are encouraged to apply for an immediately available postdoctoral position at Complutense University of Madrid, Spain. The candidate will join the LiquOrg ERC Synergy network (Pr. James Rothman, Pr. Vivek Malhotra, Pr. Frédéric Pincet and Dr. Iván López Montero). The position covers a minimum of 2-year period. We seek highly qualified applicants with experience in Atomic Force Microscopy of protein-protein and lipid- protein interactions of reconstituted systems. The LiquOrg ERC network aims to decipher the formation and self-organization of the protein secretory pathway. For more details, interested applicants should submit a brief CV and a motivation letter to Iván López-Montero (ivanlopez@quim.ucm.es)
 
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Page mise à jour le 25/11/2022
 
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