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Post-doct: Flow NMR for exploring photosynthetic enzymatic regulation on model microalgae
Bioénergétique et Ingénierie des Protéines — Marseille

Context: We are expert enzymologists and are working on microalgae. We are currently developing the use of NMR to monitor in real-time the physico-chemical and molecular transitions upon the dark/light transition at the origin of the regulation of CO2 assimilation by microalgae. The group has described the molecular mechanism responsible for the dark/light regulation of photosynthesis. We have described the molecular transitions (redox potential, structural transitions) of the intrinsically disordered protein CP12 at the origin of this regulation using NMR. Nevertheless, these effects remain challenging to monitor in real-time, and our preliminary results strongly indicate that flow-NMR is the appropriate tool to allow such study. NMR has been used to study fermentation regulation in-situ, but the study of photosynthesis regulation requires that the algae are maintained in aerobic, non-limiting [CO2] and light conditions. These conditions will be provided by newly acquired flow-NMR systems.
In the context of the recently funded ANR PhotOxIN project, we are recruiting a highly motivated post-doc to monitor in real time the structural transition of CP12, as well as the production/consumption of key photosynthesis metabolites upon a dark/light transition on cell extract or intact microalgae in aerobic and non-limiting [CO2] conditions. Different mutant strains of algae are available in the lab. The post-doc will develop the flow-NMR, and have a routine access to a 600 MHz spectrometer equipped with a cryo-probe, and access to different algal cultures.
The post-doc will benefit from our network of collaborator experts in NMR metabolomic (Mehdi Yemloul, ISM2, Marseille, France) and in NMR methods development and flow-NMR (Jonathan Farjon, CEISAM, Nantes, France), and several visiting periods in these labs are planned to ease the transfer of technology.
Profile: Candidates shall have a Ph. D. in (Bio)Chemical analysis or Chemical Physics and have demonstrated a solid track record in NMR. She/he should be open-minded due to the interdisciplinary and collaborative aspects of the project. She/he will present her/his work in national and international conferences and good skills in English are required. She/he will be highly encouraged to participate in academic (publications, conferences) and non-academic valorization (dissemination). Candidates shall have good skills and master the use of flow-NMR. Expertise on NMR methods development is welcomed. Knowledge on enzymology, photosynthesis or protein structure will be considered as a plus. Position available from Spring 2023, actual funding is for 2 years, possibility to envisage funding beyond this period. The net monthly salary is between 2000 € and 2500 €.
Application procedure: Candidates shall submit their complete Curriculum Vitae, a motivation letter as well as two recommendation letters to:
Site WebAnnonce déposée le 12/01/2023

Opening for a full-time assistant professorship in molecular and structural biology at the Department of Biology of École Polytechnique — Palaiseau (Essonne)

École Polytechnique is a leading French institute that combines top-level research, academics, and innovation at the cutting-edge of science and technology. Ecole Polytechnique has more than 3,000 students (41% of whom are international), 480 professors/researchers and 23 laboratories. Its curriculum promotes a culture of excellence with a strong emphasis on science, anchored in humanist traditions. The school aims to produce socially responsible professionals who excel in leading complex and innovative projects to address current and future challenges facing our society. École Polytechnique is a member of the “Institut Polytechnique de Paris”. It is also part of the vibrant Saclay science cluster just south of Paris. These organizations are combining their strengths to develop the excellence of research.

Teaching: The successful applicant will contribute to the teaching programs of the Department of Biology, including courses at the graduate and undergraduate levels covering molecular/structural biology and biochemistry, with applications to health and biotechnology. In particular, the assistant professor will be involved in experimental teaching for Ecole Polytechnique second and third year students. Teaching at the M2 level is also expected, including initiation to cryo-EM techniques (data collection and processing).
Research: Research will be in the Structural Biology of the Cell laboratory (BIOC), a CNRS-Ecole Polytechnique research unit that benefits from an exceptional infrastructure. The successful applicant will join the group of Emmanuelle Schmitt and Yves Mechulam specialized in the study of the molecular mechanisms of translation. The team applies a wide range of biochemical and biophysical techniques, including cryo-electron microscopy, to address fundamental questions related to ribosomal translation and its role in health and disease. The laboratory has privileged access to the CIMEX (Interdisciplinary Center of Electron Microscopy of X) which has a Titan Themis 300kV microscope equipped with a Falcon 3 direct electron detector. https://portail.polytechnique.edu/bioc/en/recherche/translation-mechanisms

Applicants should send their CV, list of publications, a short description of achievements, and contact details for three references to:
  • Thomas Simonson, Chair, Dept of biology; thomas.simonson@polytechnique.edu
  • Emmanuelle Schmitt, CNRS senior scientist; emmanuelle.schmitt@polytechnique.edu

Lire les informationsAnnonce déposée le 12/01/2023

The TATA-box Binding Protein (TBP) is universally required for the synthesis of RNA molecules encoded by eukaryotic genes. For RNA polymerase II (Pol II)-dependent genes, the interaction of TBP with the gene promoter initiates the assembly of a transcription Pre- Initiation Complex (PIC) that positions the enzyme over the transcription start site (TSS) in order to start mRNA synthesis. The multi- subunit TFIID and SAGA complexes share the capacity to load TBP onto Pol II gene promoters and enable transcription. However, how SAGA and TFIID deliver TBP to promoters, the kinetics of TBP-DNA interactions and the modes of TBP reloading are not well understood, despite the importance of these events in gene expression.

This project combines the expertise of 3 labs in molecular biology and biochemical assays, yeast genetics, kinetic measurements and structural biology, to address the role of SAGA in activated transcription initiation. The combination of in vitro reconstitution experiments, single particle cryo electron microscopy (cryo-EM) and FRET analysis aims not only at identifying key functional intermediates but also at understanding their dynamic relationships.
The postdoctoral researcher joining our team will be in charge of designing and implementing ensemble fluorescence and single molecule (sm)FRET experiments to monitor the conformational changes occurring during TBP release. Experiments include fast mixing fluorescence anisotropy to measure the binding of promoter DNA to the TBP-SAGA complex and the dissociation of SAGA. Correlation with DNA bending will be performed using smFRET on doubly labeled DNA introduced in SAGA-TBP complexes. Conformational changes within the complex will also be monitored by measuring distance changes between unnatural aminoacids introduced at key positions identified on our CryoEM structures. smFRET measurements will report on the conformational changes occurring within the complex before and during TBP release after addition of unlabeled TFIIA and DNA.

The CBS (http://www.cbs.cnrs.fr) is an institute dedicated to research at the forefront of structural biology and biophysics, with state-of-the-art facilities. Montpellier is a stunning city on the Mediterranean coast with a significant international community. Our research team includes 18 people with 8 permanent researchers. The team has a longstanding record in the training of young apprentices and mentoring young researcher. More info : https://integrativebiophysicsofmembranes.wordpress.com
The application must be submitted in English to margeat@cbs.cnrs.fr and must include the following:
  • Curriculum vitae with a list of publications,
  • A report on previous research
  • The names, addresses and contact details of 2 referees
  • All relevant diplomas, including grades
Deadline for application is February 1st, 2023. All applicants will be notified whether their application has been selected for an interview (seminar + personal visit or zoom chat).
Site WebAnnonce déposée le 02/12/2022

Page mise à jour le 12/01/2023
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